Anti-TLR5 antibody [85B152.5] (Phycoerythrin) (ab45119)

Overview

  • Product nameAnti-TLR5 antibody [85B152.5] (Phycoerythrin)
    See all TLR5 primary antibodies
  • Description
    Mouse monoclonal [85B152.5] to TLR5 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to a portion of human TLR5.

  • Positive control
    • Ramos or ThP1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab45119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 97 kDa.
Flow Cyt Use 0.5µg for 101 cells. Fixation might improve staining.



ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in diseaseSystemic lupus erythematosus 1
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody [85B152.5] (Phycoerythrin) images

  • ab45119 used at 5 µg/106 cells staining ThP1 cells by intracellular FACS analysis. The shaded histogram represents ThP1 cells alone, green represents isoytpe control and pink represents TLR5 antibody.

References for Anti-TLR5 antibody [85B152.5] (Phycoerythrin) (ab45119)

This product has been referenced in:
  • Correale J & Farez M Helminth Antigens Modulate Immune Responses in Cells from Multiple Sclerosis Patients through TLR2-Dependent Mechanisms. J Immunol 183:5999-6012 (2009). IHC-P, Flow Cyt ; Human . Read more (PubMed: 19812189) »
  • François S  et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad. J Immunol 174:3633-42 (2005). Read more (PubMed: 15749901) »

See all 2 Publications for this product

Product Wall

Thank you for your call last week, and I sincerely apologize for the delay in getting back to you.

Ihave justreceived more information about using ab45119 in flow cytometry with a surface staining protocol, and this protocol is attached to th...

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Code de remise : 100%ABR-T7TGM Date d’expiration :  18. April 2012 Je suis ravie que vous souhaitiez participer a notre offre et tester ab45119 chez le macaque. Le code de réduction correspondant est 100%ABR-T7TGM. Ce code de réduction est utilisable s...

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I am writing to follow up on an issue with two of our products, the TLR5 antibody ab45119 and the Nanog antibody ab80892. According to our accounting department, these antibodies were purchased on orders *** and *** but were not working well for you, a...

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Antibody could be used with and without fixation. Fixation could be done to compare the intra- and extracellular TLR5 presence. I have changed the wording in the application notes. To further clarify, whereas TLR5 is a cell surface receptor, TLRs...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"