Anti-TLR5 antibody (ab13868)


  • Product nameAnti-TLR5 antibody
    See all TLR5 primary antibodies
  • Description
    Rabbit polyclonal to TLR5
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 300-350 of Human TLR5.

  • Positive control
    • Ramos and Raw cell lysate.



Our Abpromise guarantee covers the use of ab13868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in diseaseSystemic lupus erythematosus 1
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody images

  • All lanes : Anti-TLR5 antibody (ab13868) at 2 µg/ml

    Lane 1 : Ramos whole cell lysate
    Lane 2 : Raw whole cell lysate
    Lane 3 : Mouse spleen whole cell lysate
    Lane 4 : Mouse lung whole cell lysate
    Lane 5 : Rat lung whole cell lysate

    Predicted band size : 103 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
  • An isotype control (top) and ab13868 (bottom), at 10 µg/ml, staining TLR5 in Human Stomach tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using ab13868 at 2 ug/106 cells. Shaded histogram represents cells without antibody; green represents a rabbit IgG isotype control; red represents anti-TLR5 antibody. Goat anti-rabbit PE (ab47646) was used as secondary antibody.

References for Anti-TLR5 antibody (ab13868)

ab13868 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cynomolgus Monkey Tissue lysate - whole (macaque neural retina)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 300 µg
Specification macaque neural retina
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization No
Specification macaque retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 04 2016

Thank you very much for your reply.

I've heard back from the lab about ab62460, and this antibody also will not work with your variant, so I am glad to hear that you've found an anti-goat secondary antibody to use with ab1654. I'm sending a f...

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Thank you very much for your call yesterday and for letting us know about the trouble with ab13868.

I've heard back from the lab about ab13875, and this antibody won't be a good choice for your truncated TLR5 variant. Theonly antibody that is...

Read More

Thank you for calling Abcam today.

I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2...

Read More