Anti-TLR5 antibody (ab37071)
Key features and details
- Rabbit polyclonal to TLR5
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TLR5 antibody
See all TLR5 primary antibodies -
Description
Rabbit polyclonal to TLR5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to 15 amino acids near the carboxy terminus or human TLR5.
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Positive control
- THP 1 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Active human TLR5 peptide (ab40018).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Active human TLR5 peptide (ab40018). |
Target
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Function
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
Involvement in disease
Systemic lupus erythematosus 1 -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Post-translational
modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7100 Human
- Omim: 603031 Human
- SwissProt: O60602 Human
- Unigene: 604542 Human
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Alternative names
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
Images
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Lane 1 : Anti-TLR5 antibody (ab37071) at 0.5 µg/ml
Lane 2 : Anti-TLR5 antibody (ab37071) at 1 µg/ml
Lane 3 : Anti-TLR5 antibody (ab37071) at 2 µg/ml
All lanes : THP 1 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
ab37071 at 10µg/ml staining TLR5 in THP-1 cells by ICC/IF
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Immunofluorescence of TLR5 in THP1 cells using ab37071 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab37071 has been referenced in 1 publication.
- Harbuzariu A et al. Neuregulin-1/ErbB4 signaling modulates Plasmodium falciparum HRP2-induced damage to brain cortical organoids. iScience 25:104407 (2022). PubMed: 35663028