Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR5
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the carboxy terminus or human TLR5.

  • Positive control
    • THP 1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab37071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Active human TLR5 peptide (ab40018).

Target

  • Function
    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in disease
    Systemic lupus erythematosus 1
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Images

  • Lane 1 : Anti-TLR5 antibody (ab37071) at 0.5 µg/ml
    Lane 2 : Anti-TLR5 antibody (ab37071) at 1 µg/ml
    Lane 3 : Anti-TLR5 antibody (ab37071) at 2 µg/ml

    Lane 1 : THP 1 cell lysate
    Lane 2 : THP 1 cell lysate
    Lane 3 : THP 1 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab37071 at 10µg/ml staining TLR5 in THP-1 cells by ICC/IF

  • Immunofluorescence of TLR5 in THP1 cells using ab37071 at 10 ug/ml.

References

ab37071 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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