The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
Use a concentration of 10 µg/ml.
FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Involvement in diseaseSystemic lupus erythematosus 1
Sequence similaritiesBelongs to the Toll-like receptor family. Contains 22 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain.
Post-translational modificationsPhosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TLR5 antibody (ab62460)Image courtesy of Dr Ilka Werner-Martini by Abreview
ab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
Zheng B et al. Transcriptional modulation of pattern recognition receptors in acute colitis in mice. Biochim Biophys Acta1832:2162-2172 (2013).
Read more (PubMed: 23851050) »
Rydberg C et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology128:e600-11 (2009).
Read more (PubMed: 19740321) »