Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR5
  • Specificity
    This antibody reacts with TLR5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide near the center of human TLR5

  • Positive control
    • Rat brain lysate, brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in disease
    Systemic lupus erythematosus 1
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody images

  • Lane 1 : Anti-TLR5 antibody (ab62460) at 0.5 µg/ml
    Lane 2 : Anti-TLR5 antibody (ab62460) at 1 µg/ml
    Lane 3 : Anti-TLR5 antibody (ab62460) at 2 µg/ml

    Lane 1 : Rat brain cell lysate
    Lane 2 : Rat brain cell lysate
    Lane 3 : Rat brain cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
  • ab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References for Anti-TLR5 antibody (ab62460)

This product has been referenced in:
  • Jiang C  et al. The Expression of Toll-like receptors in eutopic and ectopic endometrium and its implication in the inflammatory pathogenesis of adenomyosis. Sci Rep 7:7365 (2017). Read more (PubMed: 28779087) »
  • Feng Y  et al. Role of Toll-like receptors in diabetic renal lesions in a miniature pig model. Sci Adv 1:e1400183 (2015). Read more (PubMed: 26601192) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Hepatocarcinoma (HCC))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6
Permeabilization
No
Specification
Hepatocarcinoma (HCC)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (7,5 % gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic)
Specification
pancreatic
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval pH9
Permeabilization
No
Username

Dr. Ilka Werner-Martini

Verified customer

Submitted Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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