Overview

  • Product nameAnti-TLR5 antibody
    See all TLR5 primary antibodies
  • Description
    Rabbit polyclonal to TLR5
  • SpecificityThis antibody reacts with TLR5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide near the center of human TLR5

  • Positive control
    • Rat brain lysate, brain tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in diseaseSystemic lupus erythematosus 1
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody images

  • Lane 1 : Anti-TLR5 antibody (ab62460) at 0.5 µg/ml
    Lane 2 : Anti-TLR5 antibody (ab62460) at 1 µg/ml
    Lane 3 : Anti-TLR5 antibody (ab62460) at 2 µg/ml

    Lane 1 : Rat brain cell lysate
    Lane 2 : Rat brain cell lysate
    Lane 3 : Rat brain cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Immunohistochemistry of TLR5 on paraffin embedded rat brain tissue section with ab62460 at 10 µg/ml.
  • ab62460 staining TLR5 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/50 dilution for 14 hours at 4°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References for Anti-TLR5 antibody (ab62460)

This product has been referenced in:
  • Zheng B  et al. Transcriptional modulation of pattern recognition receptors in acute colitis in mice. Biochim Biophys Acta 1832:2162-2172 (2013). IHC-P ; Mouse . Read more (PubMed: 23851050) »
  • Rydberg C  et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology 128:e600-11 (2009). IHC-P ; Human . Read more (PubMed: 19740321) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Hepatocarcinoma (HCC))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6
Permeabilization No
Specification Hepatocarcinoma (HCC)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Non-reduced Denaturing (7,5 % gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreatic)
Specification pancreatic
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval pH9
Permeabilization No
Username

Dr. Ilka Werner-Martini

Verified customer

Submitted Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"