Anti-TLR7 antibody [4F4] (ab28048)
Key features and details
- Mouse monoclonal [4F4] to TLR7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-TLR7 antibody [4F4]
See all TLR7 primary antibodies -
Description
Mouse monoclonal [4F4] to TLR7 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TLR7 aa 450-550.
Database link: Q9NYK1 -
Positive control
- Ramos cell lysates
-
General notes
This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4F4 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 121 kDa.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 121 kDa. |
Target
-
Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 27 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
-
Database links
- Entrez Gene: 51284 Human
- Omim: 300365 Human
- SwissProt: Q9NYK1 Human
- Unigene: 659215 Human
-
Alternative names
- PRO285 antibody
- TLR 7 antibody
- Tlr7 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab28048 has been referenced in 3 publications.
- Makni-Maalej K et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . PubMed: 23002436
- Wang K et al. Correlation of TLR1-10 expression in peripheral blood mononuclear cells with chronic hepatitis B and chronic hepatitis B-related liver failure. Hum Immunol 71:950-6 (2010). WB ; Human . PubMed: 20667457
- Gregory SM et al. Toll-like receptor signaling controls reactivation of KSHV from latency. Proc Natl Acad Sci U S A 106:11725-30 (2009). WB ; Human . PubMed: 19564611