• Product nameAnti-TLR7 antibody
    See all TLR7 primary antibodies
  • Description
    Rabbit polyclonal to TLR7
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 706-728 of Human TLR7.

  • Positive control
    • Human Ramos cells, Raw cells.



Our Abpromise guarantee covers the use of ab13730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use at a concentration of 2 - 5 µg/1 x 106cells (Ramos or Raw cells).
    WB: Use at a concentration of 1 - 3 µg/ml for raw cell lysate. Predicted molecular weight: 121 kDa. In human Ramos cells and Raw cells, additional bands are seen (Raw cells the 121 kDa band is seen plus another lower molecular weight band).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificityDetected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • Sequence similaritiesBelongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links
    • Alternative names
      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Anti-TLR7 antibody images

    • Predicted band size : 121 kDa

      Western blot analysis of TLR7 in raw cell lysate using ab13730 at 1µg/ml.

    References for Anti-TLR7 antibody (ab13730)

    ab13730 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam with your Abvote. I would be very interested in further information as to the non-specificity that you have observed using this antibody. This antibody has only been tested using western blotting and FACS. We have the ...

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    Thank you for your enquiry. Here are some further details of the extraction buffers that I was referring to that should enable you to extract membrane bound proteins. 1. RIPA buffer (RadioImmunoPrecipitation Assay) buffer: RIPA buffer contains the ...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with ab13730 against TLR7. Given that you are obtaining a good result with your beta actin loading control antibody but not from your anti-TLR7 antibody can I just...

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    Thank you for your enquiry. Here is a protocol for cell surface staining: 1) Determine the number of cells required for staining. For each test sample, 1 x 106 cells needed. Include unstained cells, cells with a negative control and cells with p...

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    Thank you for your enquiry regarding ab13730. I have contacted the source of this antibody to find out if there is a specific recommended protocol for FACS/intracellular staining. ab7007 should work well in conjunction with this antibody. We have a...

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