Overview

  • Product nameAnti-TLR7 antibody
    See all TLR7 primary antibodies
  • Description
    Rabbit polyclonal to TLR7
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    LSHNQLTTVPERLSNCSRSLKNL

    conjugated to KLH, corresponding to amino acids 706-728 of Human TLR7

  • Positive control
    • Ramos or RAW

Properties

Applications

Our Abpromise guarantee covers the use of ab45371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
Flow Cyt Use 2-5µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityDetected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • PRO285 antibody
    • TLR 7 antibody
    • Tlr7 antibody
    • TLR7_HUMAN antibody
    • Toll like receptor 7 antibody
    • Toll-like receptor 7 antibody
    • UNQ248 antibody
    see all

Anti-TLR7 antibody images

  • Anti-TLR7 antibody (ab45371) at 1 µg/ml + Ramos whole cell lysate (ab3955)

    Predicted band size : 121 kDa
    Observed band size : 121 kDa
    Additional bands at : 200 kDa,50 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) and RAW cells (B) using ab45371 at 2 ug/ml. Histogram C shows the rabbit IgG isotype control.
  • Immunohistochemical analysis of human colon tissue using ab45371 (top) and an isotype control (bottom) at 5 ug/ml.

References for Anti-TLR7 antibody (ab45371)

This product has been referenced in:
  • Tao Y  et al. The role of endogenous IFN-ß in the regulation of Th17 responses in patients with relapsing-remitting multiple sclerosis. J Immunol 192:5610-7 (2014). Human . Read more (PubMed: 24850724) »
  • Chen GY  et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22704844) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer
Sample Sheep Tissue sections (Brain tissue (cortex))
Specification Brain tissue (cortex)
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Dendritic cells)
Loading amount 10 µg
Specification Dendritic cells
Treatment 100 nano molar LPS 24 hrs then 2 micro molar Polyvec dAdT for 4grs
Gel Running Conditions Reduced Denaturing (4-15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2013

Thank you for contacting us.

We do notsell blocking peptide for ab12121 so I am sorry we will not be able to provide this.

I have been waiting an answer from lab regarding ab13915 and ab45371; I will send you the info soon.

I...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Loading amount 35 µg
Specification Fibroblasts
Gel Running Conditions Reduced Denaturing (7% Tris-Acetat)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Lukas Rambousek

Verified customer

Submitted Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"