Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR7
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    LSHNQLTTVPERLSNCSRSLKNL

    conjugated to KLH, corresponding to amino acids 706-728 of Human TLR7

  • Positive control
    • Ramos or RAW

Properties

Applications

Our Abpromise guarantee covers the use of ab45371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
Flow Cyt Use 2-5µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • PRO285 antibody
    • TLR 7 antibody
    • Tlr7 antibody
    • TLR7_HUMAN antibody
    • Toll like receptor 7 antibody
    • Toll-like receptor 7 antibody
    • UNQ248 antibody
    see all

Images

  • Anti-TLR7 antibody (ab45371) at 3 µg/ml + RAW cell lysate

    Predicted band size : 121 kDa
  • All lanes : Anti-TLR7 antibody (ab45371) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 121 kDa


    Exposure time : 30 seconds

    Image courtesy of an anonymous AbReview

    Primary incubation: 18 hours at 4°C

  • Flow cytometric analysis of human PBMCs labeling TLR7 with ab45371 at 0.5 μg/ml (pink) compared with a rabbit isotype control (green) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (shaded). 

  • Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) and RAW cells (B) using ab45371 at 2 ug/ml. Histogram C shows the rabbit IgG isotype control.
  • RAW 246.7  (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for TLR7 (green) using ab45371 at a 1/10 dilution in ICC/IF.  Nuclei were counterstained with DAPI (blue). Alpha-tubulin was counterstained with Dylight 550 (red). 

  • Immunohistochemical analysis of human colon tissue using ab45371 (top) and an isotype control (bottom) at 5 ug/ml.

References

This product has been referenced in:
  • Hasham MG  et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). Flow Cyt . Read more (PubMed: 28250051) »
  • Jiang C  et al. The Expression of Toll-like receptors in eutopic and ectopic endometrium and its implication in the inflammatory pathogenesis of adenomyosis. Sci Rep 7:7365 (2017). Read more (PubMed: 28779087) »

See all 10 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Bouin's solution
Username

Dr. Mehmet Özbek

Verified customer

Submitted Jul 24 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (thymus, spleen)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
thymus, spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer
Sample
Sheep Tissue sections (Brain tissue (cortex))
Specification
Brain tissue (cortex)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Dendritic cells)
Loading amount
10 µg
Specification
Dendritic cells
Treatment
100 nano molar LPS 24 hrs then 2 micro molar Polyvec dAdT for 4grs
Gel Running Conditions
Reduced Denaturing (4-15)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2013

Thank you for contacting us.

We do notsell blocking peptide for ab12121 so I am sorry we will not be able to provide this.

I have been waiting an answer from lab regarding ab13915 and ab45371; I will send you the info soon.

I...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Loading amount
35 µg
Specification
Fibroblasts
Gel Running Conditions
Reduced Denaturing (7% Tris-Acetat)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Lukas Rambousek

Verified customer

Submitted Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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