Overview

  • Product nameAnti-TLR7 antibody
    See all TLR7 primary antibodies
  • Description
    Rabbit polyclonal to TLR7
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee, Monkey
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region between residues 900-950 of human TLR7 (NP_057646)

  • Positive control
    • Ramos cell lysate and human PBMC.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 121 kDa).
Flow Cyt Use 1-2µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-TLR7 antibody images

  • All lanes : Anti-TLR7 antibody (ab72542) at 1 µg/ml

    Lane 1 : Ramos cell lysate
    Lane 2 : Ramos cell lysate with immunizing peptide


    Predicted band size : 121 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
  • ab72542 at 2µg/106 cells staining TLR7 in human PBMC by flow cytometry.
    Purple histogrm is cells alone, green is rabbit IgG isotype control. Goat anti-rabbit PE was used for secondary.

References for Anti-TLR7 antibody (ab72542)

ab72542 has not yet been referenced specifically in any publications.

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We do not publish this information for all antibodies. The antibodies should detect denatured and reduced form of protein, if information is not given on the datasheet. If these does detect native form then it will be ...

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