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Overview

  • Product nameAnti-TLR8 antibody [44C143] (Phycoerythrin)
    See all TLR8 primary antibodies
  • Description
    Mouse monoclonal [44C143] to TLR8 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.

  • Positive control
    • Ramos or RAW cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab45097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa.
Flow Cyt Use 2-5µg for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityDetected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 288 antibody
      • CD288 antibody
      • CD288 antigen antibody
      • MGC119599 antibody
      • MGC119600 antibody
      • TLR 8 antibody
      • Tlr8 antibody
      • TLR8_HUMAN antibody
      • Toll like receptor 8 antibody
      • Toll-like receptor 8 antibody
      see all

    Anti-TLR8 antibody [44C143] (Phycoerythrin) images

    • Flow Cytometry / FACS - TLR8 antibody [44C143 ] (Phycoerythrin) (ab45097)
      Flow Cytometry / FACS - TLR8 antibody [44C143 ] (Phycoerythrin) (ab45097)
      ab45097 at 2µg/106 cells staining Ramos cells by flow cytometry analysis. The shaded histogram represents cells without antibody, green represents isotype control and red represents ab45097.

    References for Anti-TLR8 antibody [44C143] (Phycoerythrin) (ab45097)

    This product has been referenced in:
    • Pritchard AL  et al. Asthma is associated with multiple alterations in anti-viral innate signalling pathways. PLoS One 9:e106501 (2014). Flow Cyt ; Human . Read more (PubMed: 25203745) »
    • Young BC  et al. Longitudinal expression of Toll-like receptors on dendritic cells in uncomplicated pregnancy and postpartum. Am J Obstet Gynecol N/A:N/A (2013). Human . Read more (PubMed: 24291497) »

    See all 4 Publications for this product

    Publishing research using ab45097? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"