Overview

  • Product name
    Anti-TLR8 antibody [44C143] (Phycoerythrin)
    See all TLR8 primary antibodies
  • Description
    Mouse monoclonal [44C143] to TLR8 (Phycoerythrin)
  • Host species
    Mouse
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.

  • Positive control
    • Ramos or RAW cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab45097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa.
Flow Cyt Use 2-5µg for 106 cells.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 288 antibody
      • CD288 antibody
      • CD288 antigen antibody
      • MGC119599 antibody
      • MGC119600 antibody
      • TLR 8 antibody
      • Tlr8 antibody
      • TLR8_HUMAN antibody
      • Toll like receptor 8 antibody
      • Toll-like receptor 8 antibody
      see all

    Images

    • Flow Cytometry - Anti-TLR8 antibody [44C143] (Phycoerythrin) (ab45097)
      Flow Cytometry - Anti-TLR8 antibody [44C143] (Phycoerythrin) (ab45097)
      ab45097 at 2µg/106 cells staining Ramos cells by flow cytometry analysis. The shaded histogram represents cells without antibody, green represents isotype control and red represents ab45097.

    References

    This product has been referenced in:
    • Pritchard AL  et al. Asthma is associated with multiple alterations in anti-viral innate signalling pathways. PLoS One 9:e106501 (2014). Flow Cyt ; Human . Read more (PubMed: 25203745) »
    • Young BC  et al. Longitudinal expression of Toll-like receptors on dendritic cells in uncomplicated pregnancy and postpartum. Am J Obstet Gynecol N/A:N/A (2013). Human . Read more (PubMed: 24291497) »

    See all 4 Publications for this product

    Publishing research using ab45097? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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