Overview

  • Product nameAnti-TLR9 antibody [26C593]
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [26C593] to TLR9
  • SpecificityThis antibody is specific for TLR9.
  • Tested applicationsSuitable for: IP, IHC-Fr, IHC-P, ICC, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 268-284 of TLR9.

  • Positive control
    • Human PBMC extracts. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.

Properties

Applications

Our Abpromise guarantee covers the use of ab12121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21665946
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 117 kDa). For optimal results, primary antibody incubations should be performed at room temperature.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody [26C593] images



  • Predicted band size : 117 kDa


    TLR9 detection by Western blot. The analysis of extracts from human PBMC by using ab12121 at a dilution of 2 µg/ml.
  • ab12121 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12121 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TLR9 antibody [26C593] (ab12121)

This product has been referenced in:
  • Wang H  et al. The expression of Toll-like receptors 2, 4 and 9 in kidneys of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Clin Exp Immunol 177:603-10 (2014). Human . Read more (PubMed: 24773611) »
  • Liang Q  et al. Characterization of Sparstolonin B, a Chinese Herb-derived Compound, as a Selective Toll-like Receptor Antagonist with Potent Anti-inflammatory Properties. J Biol Chem 286:26470-9 (2011). WB, IP . Read more (PubMed: 21665946) »

See all 5 Publications for this product

Product Wall

Thank you for providing those extra details. The information provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some sugg...

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Thank you for contacting us and reporting the results you have observed with the anti-TLR9 antibody [26C593], ab12121.

In answer to your first question, as you have seen from the datasheet, this antibody has been raised against the residues 26...

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Thank you for contacting Abcam.

Below is the link to the information on quenching auto fluorescence that I mentioned:

http://www.uhnresearch.ca/facilities/wcif/PDF/Autofluorescence.pdf



Please let me know if ther...

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According to our records, ab12121 was showing some results in your hands in the ko mice. We could confirm that the ab12121 is the same clone as ab134368. However I am not sure whether this solved the problem you received.

Looking at our corr...

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Thank you for contacting us.

From the lab which producess the ab134368 Anti-TLR9 antibody [26C593.2]I got the following reply:

"Clone 26C593.2 and 26C593 is the same antibody, with and without the subclone designation. The ant...

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Thank you for contacting us.

This antibody has not been tested on knock out mice as far as I am aware. I am concerned about the information you do provide us.The specificity ofour products is most important and we take product complaints ver...

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Thank you for contacting us.

We do notsell blocking peptide for ab12121 so I am sorry we will not be able to provide this.

I have been waiting an answer from lab regarding ab13915 and ab45371; I will send you the info soon.

I...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62577.
To check the status of the order please contact our Cus...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Normal Tonsil)
Specification Human Normal Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization Yes - Methanol
Blocking step blocking agent (other company) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2009

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"