Overview

  • Product nameAnti-TLR9 antibody [5G5]
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [5G5] to TLR9
  • SpecificityThis antibody reacts very well in mouse, but more weakly in human. There are references available which suggest the antibody works well in human, however, customer feedback suggests results in human can be inconsistant.
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Dog
    Does not react with: Human
  • Immunogen

    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.

  • Positive control
    • RAW macrophages

Properties

Applications

Our Abpromise guarantee covers the use of ab16892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Flow Cyt
WB
IHC-Fr
  • Application notesFlow Cyt: 1/50.
    IHC-Fr: 1/50.Ab16892 stains RAW macrophages and TLR9 transfected HEK293 cells.
    IHC-P: Use at an assay dependent dilution.
    WB: 1/50. Predicted molecular weight: 116 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
    • Sequence similaritiesBelongs to the Toll-like receptor family.
      Contains 26 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    References for Anti-TLR9 antibody [5G5] (ab16892)

    This product has been referenced in:
    • Machida H  et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant 25:2530-537 (2010). Read more (PubMed: 20181802) »
    • Ilversaro et al Toll like receptor 9 agonists stimulate prostate cancer invasion in vitro The Prostate 67:774-781 (2007). Read more (PubMed: ) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your inquiry.

    The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as...

    Read More

    Thank you very much for your interest in our products.

    To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer ...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic)
    Specification pancreatic
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9
    Permeabilization No
    Username

    Dr. Ilka Werner-Martini

    Verified customer

    Submitted Oct 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"