Anti-TLR9 antibody [5G5] (Biotin) (ab17236)

Overview

  • Product nameAnti-TLR9 antibody [5G5] (Biotin)
    See all TLR9 primary antibodies
  • Description
    Mouse monoclonal [5G5] to TLR9 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Dog
    Does not react with: Human
  • Immunogen

    Purified fusion protein of the extracellular domain of human TLR9 (AA 1-815) and human IgGFc

  • Positive control
    • RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.

Properties

Applications

Our Abpromise guarantee covers the use of ab17236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 115 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody [5G5] (Biotin) images

  • Lane 1 : Anti-TLR9 antibody [5G5] (Biotin) (ab17236) at 1/500 dilution
    Lane 2 : TLR9 Knockout

    Lane 1 : Mouse liver whole tissue lysate
    Lane 2 : Mouse liver whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa


    Exposure time : 40 seconds

    This image is courtesy of an anonymous abreview.

    Blocking agent: 5% Milk for 1 hour at 23°C.

    See Abreview

  • Immunfluorescence analysis of RAW264.7 cells, staining TLR9 with ab17236.

    Cells were untreated (Un) or treated with GO5 or GO100 for 24 hours, fixed and then permeabilized. Cells were incubated with primary antibody (1/100) for for 1 hour at 4°C in the dark. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.
  • Flow cytometry analysis of RAW264.7 cells, staining TLR9 with ab17236.

    Cells were either untreated (green line) or treated (pink line) with GO5 or GO100 and fixed and permeabilized with 4% formaldehyde and 0.5% Tween-20. Cells were then incubated with primary antibody (1/100 dilution) for 1 hour at 4°C in the dark. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.

References for Anti-TLR9 antibody [5G5] (Biotin) (ab17236)

This product has been referenced in:
  • Chen GY  et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . Read more (PubMed: 22704844) »
  • Chen GY  et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . Read more (PubMed: 19656899) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment TLR9 knockout
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Aug 12 2015

Thank you for contacting us.

Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share i...

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Thank you for your e-mail regarding ab17236 - Mouse monoclonal [5G5] to TLR9 (Biotin). We are very sorry to hear the there are still problems with this product and have been discussing this complaint with some colleagues. We looked very carefully t...

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Thank you for your enquiry. This Ab is cited int following paper being used for WB: Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958 Conta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"