Overview

  • Product nameAnti-TLR9 antibody
    See all TLR9 primary antibodies
  • Description
    Rabbit polyclonal to TLR9
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 16 amino acids near to the C terminus of the protein (Human).

  • Positive control
    • Mouse spleen cells and cell lysate .

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21549006
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody images

  • ab37154 at 2 µg/ml staining TLR9 in mouse spleen cells by IHC.
  • Immunofluorescence of TLR9 in Mouse Spleen cells using ab37154 at 10 ug/ml.

References for Anti-TLR9 antibody (ab37154)

This product has been referenced in:
  • Hua F  et al. Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone. J Neuroinflammation 8:42 (2011). WB, IHC-FoFr ; Mouse . Read more (PubMed: 21549006) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (lung)
Gel Running Conditions Reduced Denaturing (Gel Bis-Tris 10%)
Loading amount 35 µg
Specification lung
Blocking step BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted May 18 2016

Thank you for contacting Abcam about ab37154. Please find below the suggested standard IHC-P protocol recommended for this antibody, however you may need to optimize it for your own specific needs. Standard Protocol: BUFFERS Working...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"