Overview

  • Product nameAnti-TLR9 antibody
    See all TLR9 primary antibodies
  • Description
    Rabbit polyclonal to TLR9
  • Tested applicationsSuitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the center of human TLR9

  • Positive control
    • Jurkat cells and cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody images

  • ab62577 at 2µg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.
  • Lane 1 : Anti-TLR9 antibody (ab62577) at 0.5 µg/ml
    Lane 2 : Anti-TLR9 antibody (ab62577) at 1 µg/ml
    Lane 3 : Anti-TLR9 antibody (ab62577) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 115 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of TLR9 in Mouse Spleen cells using ab62577 at 10 ug/ml.

References for Anti-TLR9 antibody (ab62577)

ab62577 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62577.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"