Overview

  • Product nameAnti-TLS/FUS antibody
    See all TLS/FUS primary antibodies
  • Description
    Rabbit polyclonal to TLS/FUS
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 1-50 of Human TLS/FUS (SwissProt: P35637).

  • Positive control
    • Human ovarian carcinoma tissue. Mouse squamous cell carcinoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab84078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 75 kDa).
ICC/IF 1/500. (see abreview).

Target

  • FunctionBinds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
    Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
    Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
    Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
  • Sequence similaritiesBelongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 75 kDa DNA pairing protein antibody
    • 75 kDa DNA-pairing protein antibody
    • ALS6 antibody
    • Amyotrophic lateral sclerosis 6 antibody
    • fus antibody
    • FUS CHOP antibody
    • Fus like protein antibody
    • FUS_HUMAN antibody
    • FUS1 antibody
    • Fused in sarcoma antibody
    • Fusion (involved in t(12;16) in malignant liposarcoma) antibody
    • Fusion derived from t(12;16) malignant liposarcoma antibody
    • Fusion gene in myxoid liposarcoma antibody
    • Heterogeneous nuclear ribonucleoprotein P2 antibody
    • hnRNP P2 antibody
    • hnRNPP2 antibody
    • Oncogene FUS antibody
    • Oncogene TLS antibody
    • POMp75 antibody
    • RNA binding protein FUS antibody
    • RNA-binding protein FUS antibody
    • TLS antibody
    • TLS CHOP antibody
    • Translocated in liposarcoma antibody
    • Translocated in liposarcoma protein antibody
    see all

Anti-TLS/FUS antibody images

  • ab84078 staining TLS/FUS in embryonic mouse motor neuron culture at 5 DIV by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton in PBS for 15 minutes and blocked with 4% BSA/4% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500: 4% BSA; 4% serum in PBS 0.1% Triton) for 16 hours at 4°C. A Cy3®-conjugated-Goat polyclonal to rabbit IgG, dilution 1/1000, was used as secondary antibody.

    See Abreview

  • All lanes : Anti-TLS/FUS antibody (ab84078) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ab84078 staining TLS/FUS in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/2000 in horse serum) for 12 hours. A Streptavidin-conjugated Horse anti-rabbit monoclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-TLS/FUS antibody (ab84078)

This product has been referenced in:
  • Shelkovnikova TA  et al. Compromised paraspeckle formation as a pathogenic factor in FUSopathies. Hum Mol Genet 23:2298-312 (2014). Read more (PubMed: 24334610) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Jim Manavis

Verified customer

Submitted Oct 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic mouse motor neuron culture at 5 DIV)
Specification embryonic mouse motor neuron culture at 5 DIV
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton in PBS 15 min
Blocking step 4% BSA, 4% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Dr. Isabelle Virard

Verified customer

Submitted Jan 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"