Overview

  • Product nameAnti-TLS/FUS antibody [EPR5812]
    See all TLS/FUS primary antibodies
  • Description
    Rabbit monoclonal [EPR5812] to TLS/FUS
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a sequence within Human TLS/FUS.

  • Positive control
    • K562, Human fetal brain, Caco 2, and HepG2 lysates; Human kidney tissue.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionBinds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseNote=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
      Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
      Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
      Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
    • Sequence similaritiesBelongs to the RRM TET family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 75 kDa DNA pairing protein antibody
      • 75 kDa DNA-pairing protein antibody
      • ALS6 antibody
      • Amyotrophic lateral sclerosis 6 antibody
      • fus antibody
      • FUS CHOP antibody
      • Fus like protein antibody
      • FUS_HUMAN antibody
      • FUS1 antibody
      • Fused in sarcoma antibody
      • Fusion (involved in t(12;16) in malignant liposarcoma) antibody
      • Fusion derived from t(12;16) malignant liposarcoma antibody
      • Fusion gene in myxoid liposarcoma antibody
      • Heterogeneous nuclear ribonucleoprotein P2 antibody
      • hnRNP P2 antibody
      • hnRNPP2 antibody
      • Oncogene FUS antibody
      • Oncogene TLS antibody
      • POMp75 antibody
      • RNA binding protein FUS antibody
      • RNA-binding protein FUS antibody
      • TLS antibody
      • TLS CHOP antibody
      • Translocated in liposarcoma antibody
      • Translocated in liposarcoma protein antibody
      see all

    Anti-TLS/FUS antibody [EPR5812] images



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TLS/FUS knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab124923 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124923 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab124923 and ab28245 (loading control to GAPDH) were both diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-TLS/FUS antibody [EPR5812] (ab124923) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    • ab124923, at 1/50 dilution, staining TLS/FUS in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-TLS/FUS antibody [EPR5812] (ab124923)

    This product has been referenced in:
    • Arnhold F  et al. Amyloid domains in the cell nucleus controlled by nucleoskeletal protein lamin B1 reveal a new pathway of mercury neurotoxicity. PeerJ 3:e754 (2015). WB . Read more (PubMed: 25699204) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"