Overview

  • Product nameAnti-TLX2 antibody
    See all TLX2 primary antibodies
  • Description
    Rabbit polyclonal to TLX2
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: QDALPRPLRP PLPPDPLCLH NSSLFALQNL QPWAEDNKVA SVSGLASVV, corresponding to C terminal amino acids 236-284 of Human TLX2

  • Positive control
    • HepG2 cell lysate, Human muscle.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

Target

  • RelevanceTLX2 contains 1 homeobox DNA-binding domain and the function remains unknown. In the mouse, this gene is expressed from embryonic day 9.5 through day 13.5 and is detectable in the dorsal root ganglia, cranial and enteric nerve ganglia and adrenal glands. In adult animals its expression is limited to the adrenal gland and the intestine. TLX2 may be involved in the proliferation and/or differentiation of enteric peripheral nervous system.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Enx antibody
    • homeo box 11 like 1 antibody
    • Homeobox protein Hox 11L1 antibody
    • Homeobox TLX 2 antibody
    • HOX11L1 antibody
    • NCX antibody
    • Neural crest homeobox protein antibody
    • PMUR10F antibody
    • T cell leukemia homeobox protein 2 antibody
    • T cell leukemia, homeobox 2 antibody
    see all

Anti-TLX2 antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-TLX2 antibody (ab51011) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab51011 at 4.0 µg/ml staining human skeletal muscle cells; paraffin embedded; magnification 400x.

References for Anti-TLX2 antibody (ab51011)

ab51011 has not yet been referenced specifically in any publications.

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