Anti-TLX3 antibody (ab97837)

Overview

  • Product nameAnti-TLX3 antibody
    See all TLX3 primary antibodies
  • Description
    Rabbit polyclonal to TLX3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human TLX3 (NP_066305)

  • Positive control
    • ACHN cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab97837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Optimal concentration of antibody depends the protein level in a given sample. Our recommended concentrations are the starting point for general use. Customers are advised have to do a series of titration tests to determine optimal concentration. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-TLX3 antibody images

  • Anti-TLX3 antibody (ab97837) at 1 µg/ml (in 5% skim milk / PBS buffer) + ACHN cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa

References for Anti-TLX3 antibody (ab97837)

ab97837 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (granule neuron progenitors)
Loading amount 20 µg
Specification granule neuron progenitors
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Thank you for contacting us. I am sorry to hear that you had problems submitting the abreview. Having had a look into it, it appears that there has been a problem with the lot number of TLX3 antibody ab97837 which is not accepted by our system. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"