Overview

  • Product name
  • Description
    Rabbit polyclonal to TM4SF3
  • Specificity
    Detects endogenous levels of total TM4SF3 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human TM4SF3

  • Positive control
    • 293 cell extract COLO cell extract HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Gastric, colon, rectal, and pancreatic carcinomas.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • anti TSPAN8 antibody
    • CO029 antibody
    • Tetraspanin 8 antibody
    • Tetraspanin-8 antibody
    • TM4SF3 antibody
    • Transmembrane 4 superfamily member 3 antibody
    • TSN8_HUMAN antibody
    • Tspan 8 antibody
    • Tspan-8 antibody
    • TSPAN8 antibody
    • TSPAN8 antibody antibody
    • Tumor associated antigen CO 029 antibody
    • Tumor-associated antigen CO-029 antibody
    see all

Images

  • All lanes : Anti-TM4SF3 antibody (ab70007) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : COLO cell extract
    Lane 3 : COLO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide.
  • IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab70007 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam regarding ab70007.


The amino acid range of this immunogen is proprietary. This antibody reacts with the second extracellular domain of the protein. We have not tested for cross reactivity with other tetras...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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