Overview

  • Product name
  • Description
    Rabbit polyclonal to TM4SF4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide designed within residues: CTGGCARCLG GTLIPLAFFG FLANILLFFP GGKVIDDNDH LSQEIWFFGG, corresponding to N terminal amino acids 2-51 of Human TM4SF4 (NP_004608)

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    TM4SF4 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TM4SF4 is a cell surface glycoprotein that regulates the adhesive and proliferative status of intestinal epithelial cells and can mediate density-dependent cell proliferation. Glycosylation is required for the growth inhibitory effect.
  • Cellular localization
    Integral to plasma membrane.
  • Database links
  • Alternative names
    • FLJ31015 antibody
    • il TMP antibody
    • ILTMP antibody
    • Intestinal and liver (il) tetraspan membrane protein antibody
    • Intestine and liver tetraspan membrane protein antibody
    • Lrtm4 antibody
    • MGC19127 antibody
    • Transmembrane 4 L six family member 4 antibody
    • transmembrane 4 L6 family member 4 antibody
    • Transmembrane 4 superfamily member 4 antibody
    see all

Images

  • Anti-TM4SF4 antibody (ab84300) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : <22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

References

ab84300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84300.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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