Overview

  • Product nameAnti-TM9SF1 antibody
    See all TM9SF1 primary antibodies
  • Description
    Rabbit polyclonal to TM9SF1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: SDRRRGDDGG FFPRTLEIHW LSIINSMVLV FLLVGFVAVI LMRVLRNDLA, corresponding to internal sequence amino acids 217-266 of Human TM9SF1

  • Positive control
    • Jurkat Cell lysate. Human Kidney.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-TM9SF1 antibody images

  • Anti-TM9SF1 antibody (ab62173) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TM9SF1 with ab62173 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-TM9SF1 antibody (ab62173)

ab62173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"