Overview

  • Product nameAnti-TM9SF1 antibody
    See all TM9SF1 primary antibodies
  • Description
    Rabbit polyclonal to TM9SF1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: KAGDPVILYV NKVGPYHNPQ ETYHYYQLPV CCPEKIRHKS LSLGEVLDGD, corresponding to N terminal amino acids 37-86 of Human TM9SF1

  • Positive control
    • Jurkat cells. Kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab62222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-TM9SF1 antibody images

  • Anti-TM9SF1 antibody (ab62222) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TM9SF1 with ab62222 at 4-8µg/ml. Arrow indicates positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-TM9SF1 antibody (ab62222)

ab62222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"