Overview

  • Product nameAnti-TMA16 antibody
    See all TMA16 primary antibodies
  • Description
    Mouse monoclonal to TMA16
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TMA16 aa 1-203. Recombinant full length protein, corresponding to amino acids 1-203 of Human FLJ11184 with a proprietary 26 kDa tag (AAH11842).

  • Positive control
    • Human breast tissue; FLJ11184 transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.

Target

  • Sequence similaritiesBelongs to the UPF0534 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • C4orf43 antibody
    • Chromosome 4 open reading frame 43 antibody
    • FLJ5295 antibody
    • Hypothetical protein FLJ11184 antibody
    • Hypothetical protein LOC55319 antibody
    • TMA16 antibody
    • TMA16_HUMAN antibody
    • Translation machinery-associated protein 16 antibody
    • UPF0534 protein C4orf43 antibody
    see all

Anti-TMA16 antibody images

  • Anti-TMA16 antibody (ab118375) at 5 µg/ml + Recombinant immunogen at 0.2 µg

    Predicted band size : 24 kDa
    Western blot against tagged recombinant immunogen using ab118375 antibody at 5µg/ml dilution. Predicted band size of immunogen is 48.44kDa.
  • All lanes : Anti-TMA16 antibody (ab118375) at 5 µg/ml

    Lane 1 : 293T cell lysate, transfected with FLJ11184
    Lane 2 : 293T cell lysate, non-transfected

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 24 kDa
  • ab118375 at 3µg/ml staining FLJ11184 in Formalin-fixed, Paraffin-embedded Human Breast tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged FLJ11184 is 0.3 ng/ml as a capture antibody.

References for Anti-TMA16 antibody (ab118375)

ab118375 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"