Overview

  • Product name
  • Description
    Rabbit polyclonal to TMC6
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    a 15 amino acid synthetic peptide from near the center of human TMC6.

  • Positive control
    • human spleen tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 90 kDa).
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Relevance
    Defects in TMC6 are a cause of epidermodysplasia verruciformis, a rare autosomal recessive genodermatosis associated with a high risk of skin carcinoma that results from an abnormal susceptibility to infection by specific human papillomaviruses. Infection leads to persistent wart-like or macular lesions. TMC6 is expressed in placenta, prostate, testis, activated T-lymphocytes and lymphokine-activated killer (LAK) lymphocytes. There are four named isoforms.
  • Cellular localization
    Endoplasmic reticulum membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • Epidermodysplasia verruciformis protein 1 antibody
    • EV1 antibody
    • EVER1 antibody
    • EVIN1 antibody
    • LAK 4P antibody
    • LAK4P antibody
    • Protein LAK 4 antibody
    • Protein LAK4 antibody
    • Transmembrane channel like protein 6 antibody
    see all

Images

  • Lane 1 : Anti-TMC6 antibody (ab67326) at 1 µg/ml
    Lane 2 : Anti-TMC6 antibody (ab67326) at 2 µg/ml

    Lane 1 : human spleen tissue lysate
    Lane 2 : human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 90 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab67326 at 2.5 µg/ml staining TMC6 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of EVER1 in Human Spleen cells using ab67326 at 20 ug/ml.

References

This product has been referenced in:
  • Lazarczyk M  et al. EVER proteins, key elements of the natural anti-human papillomavirus barrier, are regulated upon T-cell activation. PLoS One 7:e39995 (2012). WB . Read more (PubMed: 22761942) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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