Overview

  • Product name
  • Description
    Rabbit polyclonal to TMED4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    A region within synthetic peptide: MAGVGAGPLR AMGRQALLLL ALCATGAQGL YFHIGETEKR CFIEEIPDET, corresponding to N terminal amino acids 1-50 of Human TMED4

  • Positive control
    • WB: HepG2 cell lysate IHC-P: Human kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab50634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis.
  • Sequence similarities
    Belongs to the EMP24/GP25L family.
    Contains 1 GOLD domain.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum stress-response protein 25 antibody
    • ERS25 antibody
    • GMP25iso antibody
    • HNLF antibody
    • p24 family protein alpha-3 antibody
    • p24alpha3 antibody
    • Putative NF-kappa-B-activating protein 156 antibody
    • Putative NFkB activating protein HNLF antibody
    • TMED4 antibody
    • TMED4_HUMAN antibody
    • Transmembrane emp24 domain containing protein 4 antibody
    • Transmembrane emp24 domain-containing protein 4 antibody
    • Transmembrane emp24 protein transport domain containing 4 antibody
    see all

Images

  • Lane 1 : Marker
    Lane 2 : Anti-TMED4 antibody (ab50634) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysates at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • IHC-P: Human Kidney Cells with positive lable: epithelial cells of renal tubule Conc. of ab50634: 4.0 - 8.0 µg/ml

References

ab50634 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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