Anti-TMEM109 antibody (ab121349)

Overview

  • Product nameAnti-TMEM109 antibody
  • Description
    Rabbit polyclonal to TMEM109
  • Tested applicationsSuitable for: IHC-Fr, ICC, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: LEAKVRGLER QVEELRWRQR RAAKGARSVE E, corresponding to amino acids 212-242 of Human TMEM109.

  • Positive control
    • Human Pancreas tissue; U 251 MG, RT4 lysate, Human Liver and Tonsil lysates; U 251 MG cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using Fixation/Permeabilization: PFA/Triton X-100.

Target

Anti-TMEM109 antibody images

  • ab121349, at 1/1000, staining TMEM109 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
    Note: strong cytoplasmic positivity in exocrine glandular cells.
  • ab121349 at 4 µg/ml staining TMEM109 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
    Note: positivity in nucleus but not nucleoli, nuclear membrane and cytoplasm.
  • All lanes : Anti-TMEM109 antibody (ab121349) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-TMEM109 antibody (ab121349)

ab121349 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate)
Loading amount 25 µg
Specification Prostate
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate cell line LNCaP)
Specification Prostate cell line LNCaP
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2012

Thank you for contacting us.

The antibody has been tested in WB, ICC/IF and IHC-P with human samples. This antibody is fully guaranteed to be used in these listed applications and species.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"