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Rabbit polyclonal to TMEM119
Recombinant fragment corresponding to Human TMEM119 aa 117-167.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 40% Glycerol, 59% PBS
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Membrane; Single-pass type I membrane protein
Osteoblast induction factor antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM119 antibody (ab185337) Fig S3: Doi: 10.1038/mp.2016.220
Ab185337 staining TMEM119 in human induced microglia by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes. Samples were incubated with primary antibody (1:200) for 2 hours at room temperature. Hoechst nuclear stain (blue) was used at 1:5000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody (ab185337)
Immunohistochemistry analysis of Human tonsil tissue labeling TMEM119 with ab185337 at 1/50 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"