Anti-TMEM132E antibody - C-terminal (ab170376)

Overview

  • Product name
    Anti-TMEM132E antibody - C-terminal
  • Description
    Rabbit polyclonal to TMEM132E - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TMEM132E aa 711-741 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6IEE7

  • Positive control
    • MCF7 cell lysate; Mouse testis tissue lysate; MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170376 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 107 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Involvement in disease
    Note=TMEM132E is located in a region involved in a heterozygous deletion of approximately 4.7 Mb; this deletion, involving the NF1 gene and contiguous genes lying in its flanking regions, is observed in a patient 17q11.2 microdeletion syndrome, a syndrome characterized by variable facial dysmorphism, mental retardation, developmental delay, and an excessive number of neurofibromas.
  • Sequence similarities
    Belongs to the TMEM132 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • T132E_HUMAN antibody
    • TMEM132E antibody
    • Transmembrane protein 132E antibody

Images

  • Anti-TMEM132E antibody - C-terminal (ab170376) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size: 107 kDa

  • Anti-TMEM132E antibody - C-terminal (ab170376) at 1/100 dilution + MCF7 cell lysate at 35 µg

    Predicted band size: 107 kDa

  • Flow cytometric analysis of MCF7 cells labeling TMEM132E with ab170376 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170376 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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