Overview

  • Product nameAnti-TMEM143 antibody
    See all TMEM143 primary antibodies
  • Description
    Rabbit polyclonal to TMEM143
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    fragment, corresponding to internal sequence amino acids 57-197 of Human TMEM143, isoform 1.

  • Positive control
    • Human colon tissue; Transfected HEK293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-TMEM143 antibody images

  • Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli.
  • ab121431 at a 1/250 dilution staining TMEM43 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
  • All lanes : Anti-TMEM143 antibody (ab121431) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : TMEM143-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)

References for Anti-TMEM143 antibody (ab121431)

ab121431 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"