Anti-TMEM166 antibody (ab216043)
Key features and details
- Rabbit polyclonal to TMEM166
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TMEM166 antibody -
Description
Rabbit polyclonal to TMEM166 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human TMEM166 (C terminal).
Database link: Q9H8M9 -
Positive control
- WB: Human liver lysate. IHC-P: Human liver cancer and thyroid cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
1/200 - 1/1000. Predicted molecular weight: 17 kDa.
|
Notes |
---|
IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/200 - 1/1000. Predicted molecular weight: 17 kDa. |
Target
-
Function
Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. -
Tissue specificity
Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle. -
Sequence similarities
Belongs to the FAM176 family. -
Cellular localization
Endoplasmic reticulum membrane. Lysosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 84141 Human
- Entrez Gene: 232146 Mouse
- SwissProt: Q9H8M9 Human
- SwissProt: Q91WM6 Mouse
- Unigene: 302346 Human
- Unigene: 205854 Mouse
-
Alternative names
- EVA1A antibody
- F176A_HUMAN antibody
- FAM176A antibody
see all
Images
-
Anti-TMEM166 antibody (ab216043) at 1/550 dilution + Human liver lysate at 40 µg
Predicted band size: 17 kDa
Exposure time: 10 secondsGel: 12%SDS-PAGE.
-
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling TMEM166 with ab216043 at 1/30 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling TMEM166 with ab216043 at 1/30 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab216043 has not yet been referenced specifically in any publications.