Overview

  • Product nameAnti-TMEM16A antibody
    See all TMEM16A primary antibodies
  • Description
    Rabbit polyclonal to TMEM16A
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of human TMEM16A protein

  • Positive control
    • GIST

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF Use a concentration of 2 µg/ml. PubMed: 19819874
IHC-Fr Use at an assay dependent concentration. PubMed: 21084687

Target

  • FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similaritiesBelongs to the anoctamin family.
  • DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localizationCell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Anti-TMEM16A antibody images

  • ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab53212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Analysis of TMEM16A expression was performed in frozen liver sections (10 µm) from normal and 7-day bile duct-ligated rats and mice.
    Sections were defrosted at room temperature and fixed in 4% paraformaldehyde (1× PBS), permeabilized in PBST (1× PBS with 0.2% Triton), and blocked in 4% BSA (in PBST) for 1 hour at room temperature. Samples were then incubated with ab53212 at a 1/100 dilution, diluted in 1% BSA. Sections were incubated overnight at 4 °C and washed three times for 10 minutes each with 1× PBST at room temperature. Sections were incubated with Dylight 488 conjugated donkey anti-rabbit secondary at a 1/600 dilution for 2 hours at room temperature protected from light. Following incubation, the slides were washed in PBST at room temperature and coverslipped with Antifade gold and DAPI. Images were visualized using an Olympus IX-71 confocal microscope.

    Scale bar, 10 µm.

References for Anti-TMEM16A antibody (ab53212)

This product has been referenced in:
  • Wang XY  et al. Discrepancies between c-Kit positive and Ano1 positive ICC-SMP in the W/Wv and wild-type mouse colon; relationships with motor patterns and calcium transients. Neurogastroenterol Motil 26:1298-310 (2014). Mouse . Read more (PubMed: 25039457) »
  • Pritchard HA  et al. Inhibitory role of phosphatidylinositol 4,5-bisphosphate on TMEM16A-encoded calcium-activated chloride channels in rat pulmonary artery. Br J Pharmacol 171:4311-21 (2014). Rat . Read more (PubMed: 24834965) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 11 2016

ab53213 is the prediluted version of ab53212, otherwise they are the same product.

Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

Unfortunately we are not able to provide the blocking peptide for ab53212 at this time. I am very sorry that this product ...

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Thank you for your inquiry.

I can confirm that the IHC-Fr image was not generated by our lab but is taken from the following publication:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013035/?tool=pubmed

Please follow the a...

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Thank you for contacting us.
This image is from publication; Dutta AK et al, J Biol Chem. 2011 Jan 7;286(1):766-76. Epub 2010 Nov 1, Fig 1. DOI 10.1074/jbc.M110.164970. Customer can ask this to Author of the paper.
We also have DAPI mounting m...

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Thanks for your reply.

To calculate the 89% score, did you use the canis lupus sequence that I sent or a different dog sequence? I ran the BLAST again and the Identity score came up as 94%, so I'm not sure if we're looking at the same canine ...

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Thank you for your inquiry.


We do source this antibody from an outside lab, so the immunogen/epitope information is a bit vague. The immunogen range is between aa 100-450 of human DOG1, and the epitope is not within aa 386-400.
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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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Thank you for contacting us. Unfortunately, we do not currently have a blocking peptide for ab53212 available for purchase. The immunogen for this antibody is located in the range between amino acids 100- 450 of human TMEM16A. You may be able ...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"