Overview

  • Product nameAnti-TMEM16A antibody
    See all TMEM16A primary antibodies
  • Description
    Rabbit polyclonal to TMEM16A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (HGFVNHTLSS FNVSDFQNGT APNDPLDLGY EVQICRYKDY REPPWSENKY) of Human TMEM16A (NP_060513).

  • Positive control
    • Human fetal muscle lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab84115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.Can be blocked with TMEM16A peptide (ab207564). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similaritiesBelongs to the anoctamin family.
  • DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localizationCell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Anti-TMEM16A antibody images

  • Anti-TMEM16A antibody (ab84115) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 114 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-TMEM16A antibody (ab84115)

ab84115 has not yet been referenced specifically in any publications.

Product Wall

I have had the opportunity to review our anti-TMEM16A antibodies and have found two which will recognize the extracelluar domain of this target. http://www.abcam.com/tmem16a-antibody-ab84115.html was created from a peptide corresponding to a region...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"