Anti-TMEM16A antibody (ab84915)
Key features and details
- Rabbit polyclonal to TMEM16A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM16A antibody
See all TMEM16A primary antibodies -
Description
Rabbit polyclonal to TMEM16A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TMEM16A aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97423) -
Positive control
- This antibody gave a positive signal in Human Brain membrane tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
Target
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Function
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
Tissue specificity
Broadly expressed with higher levels in liver and skeletal muscle. -
Sequence similarities
Belongs to the anoctamin family. -
Domain
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
Cellular localization
Cell membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
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Alternative names
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
Images
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ab84915 staining TMEM16A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84915 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-TMEM16A antibody (ab84915) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Additional bands at: 28 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84915 has been referenced in 1 publication.
- Luo S et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res 33:253-264 (2021). PubMed: 34603794