Overview

  • Product nameAnti-TMEM16A antibody, prediluted
    See all TMEM16A primary antibodies
  • Description
    Rabbit polyclonal to TMEM16A, prediluted
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides of human DOG1 protein.

  • Positive control
    • GIST

Properties

  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 50mM Tris, pH 7.6
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1.
IHC-Fr Use at an assay dependent concentration. PubMed: 20686072
ICC/IF Use at an assay dependent concentration. PubMed: 20686072

Target

  • FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similaritiesBelongs to the anoctamin family.
  • DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localizationCell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Anti-TMEM16A antibody, prediluted images

  • ab53213 staining TMEM16A in human gastrointestinal stromal tumor sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with undiluted ab53213 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

References for Anti-TMEM16A antibody, prediluted (ab53213)

This product has been referenced in:
  • Xu J  et al. Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy. Skelet Muscle 5:43 (2015). WB . Read more (PubMed: 26693275) »
  • Bernstein K  et al. Calcium-activated chloride channels anoctamin 1 and 2 promote murine uterine smooth muscle contractility. Am J Obstet Gynecol 211:688.e1-10 (2014). Read more (PubMed: 24928056) »

See all 5 Publications for this product

Product Wall

1. The concentration is 1-2 ug/ml..

2. A green die is added to the buffer of this product. This color will not generate background or fluorescence.

ab53213 is the prediluted version of ab53212, otherwise they are the same product.

Thank you very much for your reply and for keeping me update.

I'm sorry to hear that this antibody still did not work after modifying the protocol. I'd be happy to send a replacement with a different antibody or issue a credit or refund. Plea...

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Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Je suis désolée d'apprendre que vous avez eu des problèmes avec le lot GR27833-1 ainsi que avec le Western blot sur tissu/cellules de souris.
Si vous souhaitez que nous regardons et investiguons ce problème ensemble, veuillez s'il vous plaît nous ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (GIST)
Specification GIST
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 14 2011

Thank you for contacting us. The immunogen is from amino acid range between 100 - 450 of human TMEM16A. I am sorry we do not have any additional immunogen information; this is the only information we have. I hope this information is helpful to y...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Brain and Lung)
Loading amount 20 µg
Specification Brain and Lung
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"