• Product name
    Anti-TMEM16A antibody, prediluted
    See all TMEM16A primary antibodies
  • Description
    Rabbit polyclonal to TMEM16A, prediluted
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptides of human DOG1 protein.

  • Positive control
    • GIST


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 50mM Tris, pH 7.6
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab53213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1.
IHC-Fr Use at an assay dependent concentration. PubMed: 20686072
ICC/IF Use at an assay dependent concentration. PubMed: 20686072


  • Function
    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity
    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities
    Belongs to the anoctamin family.
  • Domain
    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization
    Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all


  • ab53213 staining TMEM16A in human gastrointestinal stromal tumor sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with undiluted ab53213 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview


This product has been referenced in:
  • Suzuki A  et al. Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Mol Pain 12:N/A (2016). IHC . Read more (PubMed: 27030715) »
  • Lazrak A  et al. Hyaluronan mediates airway hyperresponsiveness in oxidative lung injury. Am J Physiol Lung Cell Mol Physiol 308:L891-903 (2015). Read more (PubMed: 25747964) »

See all 13 Publications for this product

Customer reviews and Q&As

1. The concentration is 1-2 ug/ml..

2. A green die is added to the buffer of this product. This color will not generate background or fluorescence.

ab53213 is the prediluted version of ab53212, otherwise they are the same product.

Thank you very much for your reply and for keeping me update.

I'm sorry to hear that this antibody still did not work after modifying the protocol. I'd be happy to send a replacement with a different antibody or issue a credit or refund. Plea...

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Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Je suis désolée d'apprendre que vous avez eu des problèmes avec le lot GR27833-1 ainsi que avec le Western blot sur tissu/cellules de souris.
Si vous souhaitez que nous regardons et investiguons ce problème ensemble, veuillez s'il vous plaît nous ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (GIST)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C

Abcam user community

Verified customer

Submitted Dec 14 2011

Thank you for contacting us. The immunogen is from amino acid range between 100 - 450 of human TMEM16A. I am sorry we do not have any additional immunogen information; this is the only information we have. I hope this information is helpful to y...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (Brain and Lung)
Loading amount
20 µg
Brain and Lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 22 2010


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