Anti-TMEM16B antibody (ab113443)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TMEM16B
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from the N terminal region of Human TMEM16B, conjugated to KLH.

  • Positive control
    • Human small intestine and spleen tissues; Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 114 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
  • Tissue specificity
    Retina, especially in the photoreceptor synaptic terminals.
  • Sequence similarities
    Belongs to the anoctamin family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ano2 antibody
    • ANO2_HUMAN antibody
    • Anoctamin 2 antibody
    • Anoctamin-2 antibody
    • C12orf3 antibody
    • Chromosome 12 open reading frame 3 antibody
    • DKFZp434P102 antibody
    • Transmembrane protein 16B (eight membrane-spanning domains) antibody
    • Transmembrane protein 16B antibody
    see all

Anti-TMEM16B antibody images

  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.
  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.
  • Lane 1 : Anti-TMEM16B antibody (ab113443) at 1 µg/ml
    Lane 2 : Anti-TMEM16B antibody (ab113443) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate


    Predicted band size : 114 kDa

References for Anti-TMEM16B antibody (ab113443)

ab113443 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PBS/HCl/Borax
Permeabilization
Yes - 0.2% PBS-Tx
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon Ca)
Specification
Colon Ca
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Zytomed Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

Submitted Oct 17 2012

The laboratory that produces ab113443 has sent the alignment of the immunogen with the rat and mouse proteins. As I mentioned in my previous e-mail, the identity of the immunogen sequence is considered proprietary. Homology between human and mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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