Overview

  • Product nameAnti-TMEM16B antibody
    See all TMEM16B primary antibodies
  • Description
    Rabbit polyclonal to TMEM16B
  • SpecificityThis antibody will not cross-react with TMEM16A.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TMEM16B (N terminal).
    Database link: Q9NQ90

  • Positive control
    • Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 110 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionActs as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
  • Tissue specificityRetina, especially in the photoreceptor synaptic terminals.
  • Sequence similaritiesBelongs to the anoctamin family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ano2 antibody
    • ANO2_HUMAN antibody
    • Anoctamin 2 antibody
    • Anoctamin-2 antibody
    • C12orf3 antibody
    • Chromosome 12 open reading frame 3 antibody
    • DKFZp434P102 antibody
    • Transmembrane protein 16B (eight membrane-spanning domains) antibody
    • Transmembrane protein 16B antibody
    see all

Anti-TMEM16B antibody images

  • All lanes : Anti-TMEM16B antibody (ab91573) at 2 µg/ml

    Lane 1 : Human small intestine tissue lysate
    Lane 2 : Human small intestine tissue lysate


    Predicted band size : 110 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of TMEM16B in Human Brain cells using ab91573 at 20 ug/ml.

  • Immunohistochemical analysis of human brain tissue labelling TMEM16B with ab91573 at 5 ug/mL.

  • Lane 1 : Anti-TMEM16B antibody (ab91573) at 1 µg/ml
    Lane 2 : Anti-TMEM16B antibody (ab91573) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 110 kDa
    The lower molecular weight band may represent one of the 3 isoforms of this protein which are known to exist, or a posttranslational modification.

References for Anti-TMEM16B antibody (ab91573)

ab91573 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
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Verified customer

Submitted Jan 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"