Overview

  • Product name
  • Description
    Rabbit polyclonal to TMEM173
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human TMEM173 aa 310-360.
    Database link: Q86WV6

  • Positive control
    • WB: MCF7 (human breast adenocarcinoma cell line) and HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF; HeLa (human epithelial cell line from cervix adenocarcinoma) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab92605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21838860
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the TMEM173 family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt
  • Database links
  • Alternative names
    • endoplasmic reticulum IFN stimulator antibody
    • Endoplasmic reticulum interferon stimulator antibody
    • ERIS antibody
    • FLJ38577 antibody
    • hMITA antibody
    • hSTING antibody
    • Mediator of IRF3 activation antibody
    • MITA antibody
    • Mitochondrial mediator of IRF3 activation antibody
    • MPYS antibody
    • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
    • NET23 antibody
    • Stimulator of interferon genes antibody
    • Stimulator of interferon genes protein antibody
    • STING antibody
    • TM173_HUMAN antibody
    • Tmem173 antibody
    • Transmembrane protein 173 antibody
    see all

Images

  • All lanes : Anti-TMEM173 antibody (ab92605) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry analysis of TMEM173 expression in formalin-fixed, paraffin-embedded Human breast cancer tissue an isotype control (1) or ab92605 at 5µg/ml (2 and 3).
  • ICC/IF image of ab92605 stained HeLa cells. The cells were 4% PFA fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1 % PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92605, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488® goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Formalin-fixed paraffin-embedded human colon cancer tissue stained for TMEM173 with ab92605 at a 1/100 dilution. DAB staining.

  • All lanes : Anti-TMEM173 antibody (ab92605) at 2 µg/ml

    Lane 1 : THP-1 whole cell lysate
    Lane 2 : HT-29 whole cell lysate
    Lane 3 : U2-OS whole cell lysate
    Lane 4 : Human spleen lysate

    Secondary
    anti-rabbit HRP
    Developed using the ECL technique

    Blocked using 5% non-fat milk in TBST.

References

This product has been referenced in:
  • Li T  et al. Antitumor Activity of cGAMP via Stimulation of cGAS-cGAMP-STING-IRF3 Mediated Innate Immune Response. Sci Rep 6:19049 (2016). IF . Read more (PubMed: 26754564) »
  • Panchanathan R  et al. Identification of a negative feedback loop between cyclic di-GMP-induced levels of IFI16 and p202 cytosolic DNA sensors and STING. Innate Immun N/A:N/A (2013). Read more (PubMed: 24131791) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Endothelial)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Endothelial
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 05 2017

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hep G2)
Specification
Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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