Anti-TMEM173 antibody - C-terminal (ab189430)

Overview

  • Product name
    Anti-TMEM173 antibody - C-terminal
    See all TMEM173 primary antibodies
  • Description
    Rabbit polyclonal to TMEM173 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMEM173 aa 318-348 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    ADDSSFSLSQEVLRHLRQEEKEEVTVGSLKT


    Database link: Q86WV6

  • Positive control
    • Human tonsil tissue; HeLa cells; Mouse spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the TMEM173 family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt
  • Database links
  • Alternative names
    • endoplasmic reticulum IFN stimulator antibody
    • Endoplasmic reticulum interferon stimulator antibody
    • ERIS antibody
    • FLJ38577 antibody
    • hMITA antibody
    • hSTING antibody
    • Mediator of IRF3 activation antibody
    • MITA antibody
    • Mitochondrial mediator of IRF3 activation antibody
    • MPYS antibody
    • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
    • NET23 antibody
    • Stimulator of interferon genes antibody
    • Stimulator of interferon genes protein antibody
    • STING antibody
    • TM173_HUMAN antibody
    • Tmem173 antibody
    • Transmembrane protein 173 antibody
    see all

Images

  • Anti-TMEM173 antibody - C-terminal (ab189430) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 42 kDa

  • Flow cytometric analysis of HeLa cells labeling TMEM173 with ab189430 at 1/10 dilution (right histogram) compared to negative control cells (left histogram) using FITC-conjugated goat-anti-rabbit secondary antibodies.

     

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling TMEM173 with ab189430 at 5 μg/ml.

References

ab189430 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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