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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 17 kDa).
Function Cell migration modulator which enhances the glioma-specific migration ability of neural stem cells (NSC) and neural precursor cells (NPC).
Sequence similarities Belongs to the TMEM18 family.
Cellular localization Nucleus membrane.
Information by UniProt
Form Nucleus Membrane
Transmembrane protein 18 antibody
Anti-Tmem18 antibody images
Western blot - Tmem18 antibody (ab100954)
All lanes : Anti-Tmem18 antibody (ab100954) at 1 µg/ml Lane 1 : Human brain tissue lysate - total protein ( ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 17 kDa Observed band size : 23 kDa ( why is the actual band size different from the predicted?) Additional bands at : 45 kDa,50 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
Western blot - Anti-Tmem18 antibody (ab100954)
Anti-Tmem18 antibody (ab100954) at 1 µg/ml + Human brain tissue lysate - total protein ( ab29466) at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 17 kDa Observed band size : 24 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands. Exposure time : 2 minutes
References for Anti-Tmem18 antibody (ab100954)
has not yet been referenced specifically in any publications.
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