Recombinant
RabMAb

Anti-TMEM192 antibody [EPR14330] (ab185545)

Overview

  • Product name
    Anti-TMEM192 antibody [EPR14330]
    See all TMEM192 primary antibodies
  • Description
    Rabbit monoclonal [EPR14330] to TMEM192
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within TMEM192. The exact sequence is proprietary. Recombinant fragment (aa1-46) and (aa193-271)

  • Positive control
    • U87-MG cell & Human fetal liver lysates, HepG2 & U87-MG cells,
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14330
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100.
Flow Cyt 1/240. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Flow cytometry analysis using ab185545 at 1/240 dilution on U87-MG cells.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/250 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

  • Immunofluorescence analysis of HepG2 cells labeling TMEM192 with ab185545 at 1/100 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi.

  • Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/2000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 31 kDa

    Blocking buffer: 5% NFDM/TBST

  • Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 31 kDa

    Blocking buffer: 5% NFDM/TBST

References

ab185545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185545.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up