Overview

  • Product name
    Anti-TMEM195 antibody
  • Description
    Rabbit polyclonal to TMEM195
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 2-51 (KNPEAQQDVS VSQGFRMLFY TMKPSETSFQ TLEEVPDYVK KATPFFISLM) of Human TMEM195 (NP_001004320)

  • Positive control
    • Transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    TMEM195 is a glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes.
  • Cellular localization
    Endoplasmic reticulum membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • Alkylglycerol monooxygenase antibody
    • Transmembrane protein 195 antibody

Anti-TMEM195 antibody images

  • Anti-TMEM195 antibody (ab87236) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa

References for Anti-TMEM195 antibody (ab87236)

ab87236 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver, Colon)
Gel Running Conditions
Reduced Denaturing (10 % SDS gel)
Loading amount
50 µg
Specification
Liver, Colon
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
Username

Caroline Fischer

Verified customer

Submitted Oct 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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