Overview

  • Product name
    Anti-TMEM234 antibody
  • Description
    Rabbit polyclonal to TMEM234
  • Host species
    Rabbit
  • Specificity
    ab121459 will recognise isoform 2 of TMEM234 (Q8WY98-2)
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    DIGGKRKLDY CECGTQLCGS RHTCVSSFPE PISPEWVRTR PFPILPFPLQ LFCF, corresponding to amino acids 105-158 of isform 2 of Human TMEM234 isoform 2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human cerebral cortex tissue; RT4 cell lysate; U251 MG cell lysate; Human liver lysate; Human tonsil lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM234 antibody (ab121459)
    Immunocytochemistry/ Immunofluorescence - Anti-TMEM234 antibody (ab121459)
    Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab121459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Western blot - Anti-TMEM234 antibody (ab121459)
    Western blot - Anti-TMEM234 antibody (ab121459)
    All lanes : Anti-TMEM234 antibody (ab121459) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma cell lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.


    10-20% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM234 antibody (ab121459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM234 antibody (ab121459)
    ab121459 at 1/35 dilution staining TMEM234 in Human cerebral cortex tissue (paraffin-embedded) by immunohistochemistry.

References

ab121459 has not yet been referenced specifically in any publications.

Publishing research using ab121459? Please let us know so that we can cite the reference in this datasheet.

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