Customer reviews and FAQs

Overview

  • Product name
    Anti-TMEM234 antibody
  • Description
    Rabbit polyclonal to TMEM234
  • Specificity
    ab121459 will recognise isoform 2 of TMEM234 (Q8WY98-2)
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    DIGGKRKLDY CECGTQLCGS RHTCVSSFPE PISPEWVRTR PFPILPFPLQ LFCF, corresponding to amino acids 105-158 of isform 2 of Human TMEM234 isoform 2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human cerebral cortex tissue; RT4 cell lysate; U251 MG cell lysate; Human liver lysate; Human tonsil lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-TMEM234 antibody images

  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM234 antibody (ab121459)
    Immunocytochemistry/ Immunofluorescence - Anti-TMEM234 antibody (ab121459)
    Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab121459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Western blot - Anti-TMEM234 antibody (ab121459)
    Western blot - Anti-TMEM234 antibody (ab121459)
    All lanes : Anti-TMEM234 antibody (ab121459) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma cell lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique10-20% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM234 antibody (ab121459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM234 antibody (ab121459)
    ab121459 at 1/35 dilution staining TMEM234 in Human cerebral cortex tissue (paraffin-embedded) by immunohistochemistry.

References for Anti-TMEM234 antibody (ab121459)

ab121459 has not yet been referenced specifically in any publications.

Publishing research using ab121459? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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