Overview

  • Product name
    Anti-TMEM53 antibody
  • Description
    Rabbit polyclonal to TMEM53
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: FHTHFYDRLQ DAGSRWPELY LYSRADEVVL ARDIERMVEA RLARRVLARS VDFVSSAHVS HLRDYPTYYT SLCVDFMRNC VRC, corresponding to amino acids 195-277 of Human TMEM53.

  • Positive control
    • TMEM53 over-expression HEK293T lysate; Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and focal adhesions. Recommended concentration of ab121648 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-TMEM53 antibody (ab121648) at 1/250 dilution

    Lane 1 : vector only transfected HEK293T lysate
    Lane 2 : TMEM53 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
    10-20% SDS PAGE
  • ab121648 at 1/40 dilution staining TMEM53 in Paraffin-embedded Human colon tissue by Immunohistochemistry.

References

ab121648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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