Overview

  • Product nameAnti-TMEM66 antibody
    See all TMEM66 primary antibodies
  • Description
    Rabbit polyclonal to TMEM66
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 36-66 of Human TMEM66 (NP_057211.4).

  • Positive control
    • 293 cell line and Mouse cerebellum tissue lysates. IF/ICC: RAW 246.7 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab107694 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.

Target

Anti-TMEM66 antibody images

  • ICC/IF image of ab107694 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107694, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TMEM66 antibody (ab107694) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 37 kDa
  • Anti-TMEM66 antibody (ab107694) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size : 37 kDa

References for Anti-TMEM66 antibody (ab107694)

ab107694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"