Overview

  • Product name
  • Description
    Rabbit polyclonal to TMEM66
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-211 of Human TMEM66 (Uniprot ID: Q96BY9).

  • Positive control
    • 293T, Jurkat, Raji whole cell lysate; Human hepatoma tissue; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-TMEM66 antibody (ab154538) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size : 37 kDa
  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling TMEM66 with ab154538 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMEM66 with ab154538 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154538 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

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Verified customer

Submitted Jul 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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