Overview

  • Product nameAnti-TMEM66 antibody
    See all TMEM66 primary antibodies
  • Description
    Rabbit polyclonal to TMEM66
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (RFTNSAGPPP PGFKSEFTGP QNTGHGATSG FGSAFTGQQG YENSGPGFWT) of Human TMEM66 (NP_057211).

  • Positive control
    • Transfected 293T cell lysate. IF/ICC: RAW 246.7 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-TMEM66 antibody images

  • Anti-TMEM66 antibody (ab80890) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab80890 stained RAW 246.7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab80890, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TMEM66 antibody (ab80890)

This product has been referenced in:
  • Taha S  et al. Differential upregulation of the hypothetical transmembrane protein 66 (TMEM66) in multiple sclerosis patients with potential inflammatory response. Biomed Rep 3:98-104 (2015). WB ; Human . Read more (PubMed: 25469256) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"