Overview

  • Product name
    Anti-TMEM74 antibody
  • Description
    Rabbit polyclonal to TMEM74
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 (RLEKESARLG AHLDRCVIAG LCLLTLGGVI LSCLLMMSMW KGELYRRNRF) of human TMEM74 (NP_694560).

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. It is expressed in heart, lung and placenta.
  • Cellular localization
    Lysosome membrane; Multi pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi pass membrane protein.
  • Database links
  • Alternative names
    • Transmembrane protein 74 antibody

Images

  • Anti-TMEM74 antibody (ab87239) at 1 µg/ml + human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References

ab87239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab87239.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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