Recombinant
RabMAb

Anti-TMEM9B antibody [EPR14339] (ab189253)

Overview

  • Product name
    Anti-TMEM9B antibody [EPR14339]
    See all TMEM9B primary antibodies
  • Description
    Rabbit monoclonal [EPR14339] to TMEM9B
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Human TMEM9B aa 1-100. The exact sequence is proprietary.
    Database link: Q9NQ34

    Recombinant fragment within Human TMEM9B aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NQ34

  • Positive control
    • HeLa, HepG2, BxPC-3, Mouse brain, rat brain lysates; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14339
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).

Target

Images

  • All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 23 kDa
  • Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling TMEM9B with ab189253 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 23 kDa

References

ab189253 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189253.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up