RelevanceThe TMIE gene encodes a transmembrane inner ear protein. TMIE is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles, in mice. It is one of multiple genes responsible for recessive non-syndromic deafness (DFNB). This is also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment.
Cellular localizationMembrane; Single-pass type I membrane protein
transmembrane inner ear expressed protein antibody
transmembrane inner ear protein antibody
Anti-TMIE antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMIE antibody (ab103028)This image is courtesy of an Abreview submitted by Angela Ballesteros Morcillo
ab103028 staining TMIE in mouse COS-7 cells transfected with TMIE-cherry plasmid by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 4% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.