Overview

  • Product name
    Anti-TMIE antibody
  • Description
    Rabbit polyclonal to TMIE
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 78-108 of Human TMIE

  • Positive control
    • Mouse liver nuclear lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.

Target

  • Relevance
    The TMIE gene encodes a transmembrane inner ear protein. TMIE is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles, in mice. It is one of multiple genes responsible for recessive non-syndromic deafness (DFNB). This is also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment.
  • Cellular localization
    Membrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • deafness autosomal recessive 6 antibody
    • DFNB6 antibody
    • transmembrane inner ear antibody
    • transmembrane inner ear expressed protein antibody
    • transmembrane inner ear protein antibody
    see all

Images

  • ab103028 staining TMIE in mouse COS-7 cells transfected with TMIE-cherry plasmid by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 4% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-TMIE antibody (ab103028) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

    Predicted band size : 17 kDa

References

ab103028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (COS-7 cells)
Specification
COS-7 cells
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Angela Ballesteros Morcillo

Verified customer

Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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