Overview

  • Product nameAnti-TMIE antibody
  • Description
    Rabbit polyclonal to TMIE
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 78-108 of Human TMIE

  • Positive control
    • Mouse liver nuclear lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.

Target

  • RelevanceThe TMIE gene encodes a transmembrane inner ear protein. TMIE is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles, in mice. It is one of multiple genes responsible for recessive non-syndromic deafness (DFNB). This is also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment.
  • Cellular localizationMembrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • deafness autosomal recessive 6 antibody
    • DFNB6 antibody
    • transmembrane inner ear antibody
    • transmembrane inner ear expressed protein antibody
    • transmembrane inner ear protein antibody
    see all

Anti-TMIE antibody images

  • ab103028 staining TMIE in mouse COS-7 cells transfected with TMIE-cherry plasmid by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 4% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-TMIE antibody (ab103028) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

    Predicted band size : 17 kDa

References for Anti-TMIE antibody (ab103028)

ab103028 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (COS-7 cells)
Specification COS-7 cells
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Dr. Angela Ballesteros Morcillo

Verified customer

Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"