Overview

  • Product nameAnti-TMPRSS2 antibody [EPR3862]
    See all TMPRSS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3862] to TMPRSS2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 450-550 (C terminal).

  • Positive control
    • Human prostate tissue lysate, Human prostatic adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
WB 1/1000 - 1/5000. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.

For unpurified use at 1/500 - 1/1000.

Target

  • Tissue specificityExpressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 LDL-receptor class A domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localizationCell membrane and Secreted. Activated by cleavage and secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • D16Ertd61e antibody
    • Epitheliasin antibody
    • FLJ41954 antibody
    • MGC6821 antibody
    • PP9284 antibody
    • PRSS10 antibody
    • Serine protease 10 antibody
    • TMPRSS2 antibody
    • TMPRSS2 ERG FUSION GENE, INCLUDED antibody
    • TMPRSS2 ETV1 FUSION GENE, INCLUDED antibody
    • TMPS2_HUMAN antibody
    • Transmembrane protease serine 2 catalytic chain antibody
    • Transmembrane protease, serine 2 antibody
    • Transmembrane protease, serine 2, EC 3.4.219 antibody
    see all

Anti-TMPRSS2 antibody [EPR3862] images

  • ab109131 staining TMPRSS2 in the HT-29 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • ab109131 staining TMPRSS2 in Human prostrate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilution

    Lane 1 : Human prostrate Lysate
    Lane 2 : LnCaP Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
  • All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/1000 dilution (unpurified)

    Lane 1 : Human prostate tissue lysate
    Lane 2 : Human prostate tissue lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 54 kDa
  • Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.

References for Anti-TMPRSS2 antibody [EPR3862] (ab109131)

ab109131 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109131.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"