Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 450-550 (C terminal).
Human prostate tissue lysate, Human prostatic adenocarcinoma tissue
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
ab109131 staining TMPRSS2 in the HT-29 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.
ab109131 staining TMPRSS2 in Human prostrate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-TMPRSS2 antibody [EPR3862] (ab109131)
All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilution
Lane 1 : Human prostrate Lysate Lane 2 : LnCaP Lysate
Lysates/proteins at 20 µg per lane.
Secondary Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 54 kDa
Western blot - TMPRSS2 antibody [EPR3862] (ab109131)
All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/1000 dilution (unpurified)
Lane 1 : Human prostate tissue lysate Lane 2 : Human prostate tissue lysate